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egm-2mv medium cc-3202  (Lonza)


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    Structured Review

    Lonza egm-2mv medium cc-3202
    Egm 2mv Medium Cc 3202, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egm-2mv+medium/egm+2+medium/pmc12212142-44-0-3
    Average 90 stars, based on 1 article reviews
    egm-2mv medium cc-3202 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Control:

    Article Title: Insulin regulates lymphatic endothelial integrity via palmitoylation.
    Article Snippet: Primary human dermal lymphatic endothelial cells (hereafter referred to as LECs) were obtained from PromoCell and grown in EGM-2MV (microvascular endothelial cell growth medium-2) medium from Lonza Bioscience and used between passages 2 and 8 for experiments as previously described (6).

    Article Title: Cysteine Leukotriene Receptor Antagonist-Montelukast Effects on Diabetic Retinal Microvascular Endothelial Cells Curtail Autophagy.
    Article Snippet: Primary human retinal microvascular endothelial cells (HRECs; Cat. no. ACBRI 181; Cell Systems, Inc., Kirkland, WA, USA) were routinely cultured in EGM-2MVmedium (Cat. no. CC-4147; Lonza Group, Basel, Switzerland) in collagen coated 10 cm dishes (113 μL of 3 mg/mL in 20 mM glacial acetic acid, Cat. no. A10483-01; Life Technologies, Carlsbad, CA, USA) and incubated in a humidified chamber set at 37°C and 5% CO2.

    Article Title: Targeting Tumor Endothelial Cells by EGCG Using Specific Liposome Delivery System Inhibits Vascular Inflammation and Thrombosis
    Article Snippet: To enhance the purity of TEC, CD45−/CD31+ cells were further sorted using a FACS Aria II (BD Biosciences).

    Article Title: Neurotensin Regulates Primate Ovulation Via Multiple Neurotensin Receptors.
    Article Snippet: Cells were grown in EGM-2MV medium (Lonza).

    Article Title: Stem cell-based lung-on-chip models
    Article Snippet: Chips were performed for 24 hours using EGM-2MV medium (Lonza), while Human Primary Alveolar Epithelial Cells were kept submerged in SAGM (Lonza) 0% serum with added KIAD supplements.

    Article Title: Insulin regulates lymphatic endothelial integrity via palmitoylation
    Article Snippet: Primary human dermal lymphatic endothelial cells (hereafter referred to as LECs) were obtained from PromoCell and grown in EGM-2MV (microvascular endothelial cell growth medium-2) medium from Lonza Bioscience and used between passages 2 and 8 for experiments as previously described ( ).

    Article Title: Cuproptosis, a potential target for the therapy of diabetic critical limb ischemia.
    Article Snippet: Diabetic patients are considered as the high risk population to develop critical limb ischemia (CLI), a peripheral vascular disease (PVD) resulted from atherosclerosis.. Cuproptosis is a novel copper-dependent cell death that has shown the regulatory role in diabetes, while its effect on diabetic CLI has not been explored yet.. In this study, Diabetic CLI mice was induced by femoral artery ligation (FAL) on diabetic mice.

    Article Title: Human Dermal Microvascular Arterial and Venous Blood Endothelial Cells and Their Use in Bioengineered Dermo-Epidermal Skin Substitutes.
    Article Snippet: Cell Isolation and Culture: HDMECs (n = 3 independent biological samples; three donors) were positively isolated from human foreskins with CD31-Dynabeads (Thermo Fisher Scientific, Basel, Switzerland) according to the manufacturer’s instructions.

    Cell Culture:

    Article Title: Insulin regulates lymphatic endothelial integrity via palmitoylation.
    Article Snippet: Primary human dermal lymphatic endothelial cells (hereafter referred to as LECs) were obtained from PromoCell and grown in EGM-2MV (microvascular endothelial cell growth medium-2) medium from Lonza Bioscience and used between passages 2 and 8 for experiments as previously described (6).

    Article Title: Cysteine Leukotriene Receptor Antagonist-Montelukast Effects on Diabetic Retinal Microvascular Endothelial Cells Curtail Autophagy.
    Article Snippet: Primary human retinal microvascular endothelial cells (HRECs; Cat. no. ACBRI 181; Cell Systems, Inc., Kirkland, WA, USA) were routinely cultured in EGM-2MVmedium (Cat. no. CC-4147; Lonza Group, Basel, Switzerland) in collagen coated 10 cm dishes (113 μL of 3 mg/mL in 20 mM glacial acetic acid, Cat. no. A10483-01; Life Technologies, Carlsbad, CA, USA) and incubated in a humidified chamber set at 37°C and 5% CO2.

    Article Title: Targeting Tumor Endothelial Cells by EGCG Using Specific Liposome Delivery System Inhibits Vascular Inflammation and Thrombosis
    Article Snippet: To enhance the purity of TEC, CD45−/CD31+ cells were further sorted using a FACS Aria II (BD Biosciences).

    Article Title: Neurotensin Regulates Primate Ovulation Via Multiple Neurotensin Receptors.
    Article Snippet: Cells were grown in EGM-2MV medium (Lonza).

    Article Title: Stem cell-based lung-on-chip models
    Article Snippet: Chips were performed for 24 hours using EGM-2MV medium (Lonza), while Human Primary Alveolar Epithelial Cells were kept submerged in SAGM (Lonza) 0% serum with added KIAD supplements.

    Article Title: Insulin regulates lymphatic endothelial integrity via palmitoylation
    Article Snippet: Primary human dermal lymphatic endothelial cells (hereafter referred to as LECs) were obtained from PromoCell and grown in EGM-2MV (microvascular endothelial cell growth medium-2) medium from Lonza Bioscience and used between passages 2 and 8 for experiments as previously described ( ).

    Article Title: Cuproptosis, a potential target for the therapy of diabetic critical limb ischemia.
    Article Snippet: Diabetic patients are considered as the high risk population to develop critical limb ischemia (CLI), a peripheral vascular disease (PVD) resulted from atherosclerosis.. Cuproptosis is a novel copper-dependent cell death that has shown the regulatory role in diabetes, while its effect on diabetic CLI has not been explored yet.. In this study, Diabetic CLI mice was induced by femoral artery ligation (FAL) on diabetic mice.

    Article Title: Human Dermal Microvascular Arterial and Venous Blood Endothelial Cells and Their Use in Bioengineered Dermo-Epidermal Skin Substitutes.
    Article Snippet: Cell Isolation and Culture: HDMECs (n = 3 independent biological samples; three donors) were positively isolated from human foreskins with CD31-Dynabeads (Thermo Fisher Scientific, Basel, Switzerland) according to the manufacturer’s instructions.

    Isolation:

    Article Title: Insulin regulates lymphatic endothelial integrity via palmitoylation.
    Article Snippet: Primary human dermal lymphatic endothelial cells (hereafter referred to as LECs) were obtained from PromoCell and grown in EGM-2MV (microvascular endothelial cell growth medium-2) medium from Lonza Bioscience and used between passages 2 and 8 for experiments as previously described (6).

    Article Title: Cysteine Leukotriene Receptor Antagonist-Montelukast Effects on Diabetic Retinal Microvascular Endothelial Cells Curtail Autophagy.
    Article Snippet: Primary human retinal microvascular endothelial cells (HRECs; Cat. no. ACBRI 181; Cell Systems, Inc., Kirkland, WA, USA) were routinely cultured in EGM-2MVmedium (Cat. no. CC-4147; Lonza Group, Basel, Switzerland) in collagen coated 10 cm dishes (113 μL of 3 mg/mL in 20 mM glacial acetic acid, Cat. no. A10483-01; Life Technologies, Carlsbad, CA, USA) and incubated in a humidified chamber set at 37°C and 5% CO2.

    Article Title: Targeting Tumor Endothelial Cells by EGCG Using Specific Liposome Delivery System Inhibits Vascular Inflammation and Thrombosis
    Article Snippet: To enhance the purity of TEC, CD45−/CD31+ cells were further sorted using a FACS Aria II (BD Biosciences).

    Article Title: Neurotensin Regulates Primate Ovulation Via Multiple Neurotensin Receptors.
    Article Snippet: Cells were grown in EGM-2MV medium (Lonza).

    Article Title: Stem cell-based lung-on-chip models
    Article Snippet: Chips were performed for 24 hours using EGM-2MV medium (Lonza), while Human Primary Alveolar Epithelial Cells were kept submerged in SAGM (Lonza) 0% serum with added KIAD supplements.

    Article Title: Insulin regulates lymphatic endothelial integrity via palmitoylation
    Article Snippet: Primary human dermal lymphatic endothelial cells (hereafter referred to as LECs) were obtained from PromoCell and grown in EGM-2MV (microvascular endothelial cell growth medium-2) medium from Lonza Bioscience and used between passages 2 and 8 for experiments as previously described ( ).

    Article Title: Cuproptosis, a potential target for the therapy of diabetic critical limb ischemia.
    Article Snippet: Diabetic patients are considered as the high risk population to develop critical limb ischemia (CLI), a peripheral vascular disease (PVD) resulted from atherosclerosis.. Cuproptosis is a novel copper-dependent cell death that has shown the regulatory role in diabetes, while its effect on diabetic CLI has not been explored yet.. In this study, Diabetic CLI mice was induced by femoral artery ligation (FAL) on diabetic mice.

    Article Title: Human Dermal Microvascular Arterial and Venous Blood Endothelial Cells and Their Use in Bioengineered Dermo-Epidermal Skin Substitutes.
    Article Snippet: Cell Isolation and Culture: HDMECs (n = 3 independent biological samples; three donors) were positively isolated from human foreskins with CD31-Dynabeads (Thermo Fisher Scientific, Basel, Switzerland) according to the manufacturer’s instructions.

    Incubation:

    Article Title: Insulin regulates lymphatic endothelial integrity via palmitoylation.
    Article Snippet: Primary human dermal lymphatic endothelial cells (hereafter referred to as LECs) were obtained from PromoCell and grown in EGM-2MV (microvascular endothelial cell growth medium-2) medium from Lonza Bioscience and used between passages 2 and 8 for experiments as previously described (6).

    Article Title: Cysteine Leukotriene Receptor Antagonist-Montelukast Effects on Diabetic Retinal Microvascular Endothelial Cells Curtail Autophagy.
    Article Snippet: Primary human retinal microvascular endothelial cells (HRECs; Cat. no. ACBRI 181; Cell Systems, Inc., Kirkland, WA, USA) were routinely cultured in EGM-2MVmedium (Cat. no. CC-4147; Lonza Group, Basel, Switzerland) in collagen coated 10 cm dishes (113 μL of 3 mg/mL in 20 mM glacial acetic acid, Cat. no. A10483-01; Life Technologies, Carlsbad, CA, USA) and incubated in a humidified chamber set at 37°C and 5% CO2.

    Article Title: Targeting Tumor Endothelial Cells by EGCG Using Specific Liposome Delivery System Inhibits Vascular Inflammation and Thrombosis
    Article Snippet: To enhance the purity of TEC, CD45−/CD31+ cells were further sorted using a FACS Aria II (BD Biosciences).

    Article Title: Neurotensin Regulates Primate Ovulation Via Multiple Neurotensin Receptors.
    Article Snippet: Cells were grown in EGM-2MV medium (Lonza).

    Article Title: Stem cell-based lung-on-chip models
    Article Snippet: Chips were performed for 24 hours using EGM-2MV medium (Lonza), while Human Primary Alveolar Epithelial Cells were kept submerged in SAGM (Lonza) 0% serum with added KIAD supplements.

    Article Title: Insulin regulates lymphatic endothelial integrity via palmitoylation
    Article Snippet: Primary human dermal lymphatic endothelial cells (hereafter referred to as LECs) were obtained from PromoCell and grown in EGM-2MV (microvascular endothelial cell growth medium-2) medium from Lonza Bioscience and used between passages 2 and 8 for experiments as previously described ( ).

    Article Title: Cuproptosis, a potential target for the therapy of diabetic critical limb ischemia.
    Article Snippet: Diabetic patients are considered as the high risk population to develop critical limb ischemia (CLI), a peripheral vascular disease (PVD) resulted from atherosclerosis.. Cuproptosis is a novel copper-dependent cell death that has shown the regulatory role in diabetes, while its effect on diabetic CLI has not been explored yet.. In this study, Diabetic CLI mice was induced by femoral artery ligation (FAL) on diabetic mice.

    Article Title: Human Dermal Microvascular Arterial and Venous Blood Endothelial Cells and Their Use in Bioengineered Dermo-Epidermal Skin Substitutes.
    Article Snippet: Cell Isolation and Culture: HDMECs (n = 3 independent biological samples; three donors) were positively isolated from human foreskins with CD31-Dynabeads (Thermo Fisher Scientific, Basel, Switzerland) according to the manufacturer’s instructions.



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    Image Search Results


    Effect of 24 h hypoxia on human retinal endothelial cell (hREC) and human Müller (MIO-M1) cell proliferation and viability. In both hRECs ( A , C , E ) and MIO-M1 cells ( B , D , F ), cell proliferation was measured using the MTT assay ( A , B ), while cell viability was evaluated by the trypan blue ( C , D ) and lactate concentration measurement ( E , F ) assays. Data are shown as box plots with minimum to maximum whiskers and represent the absorbance values normalized to those measured in normoxic controls ( n = 10 in A , B , n = 5 in C – F ). Statistical significance was evaluated through unpaired t -test. ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells

    doi: 10.3390/ijms26094043

    Figure Lengend Snippet: Effect of 24 h hypoxia on human retinal endothelial cell (hREC) and human Müller (MIO-M1) cell proliferation and viability. In both hRECs ( A , C , E ) and MIO-M1 cells ( B , D , F ), cell proliferation was measured using the MTT assay ( A , B ), while cell viability was evaluated by the trypan blue ( C , D ) and lactate concentration measurement ( E , F ) assays. Data are shown as box plots with minimum to maximum whiskers and represent the absorbance values normalized to those measured in normoxic controls ( n = 10 in A , B , n = 5 in C – F ). Statistical significance was evaluated through unpaired t -test. ** p < 0.01.

    Article Snippet: hRECs constitute a primary human cell line composed of a mixture of venous and arterial cell populations [ ]. hRECs were cultured in Endothelial Basal Medium-2 (EBM-2; Lonza, Walkersville, MD, USA), supplemented with 10% Fetal Bovine Serum (FBS) and endothelial growth factors (Microvascular Endothelial Cell Growth Medium-2, EGM-2MV SingleQuot; Lonza).

    Techniques: MTT Assay, Concentration Assay

    Effect of 24 h hypoxia on hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) expression. ( A , B ) mRNA levels of VEGF in hRECs ( A ) and MIO-M1 cells ( B ). ( C – H ) Representative Western blots ( C , F ) and densitometric analysis ( D , E , G , H ) of HIF-1α ( D , G ) and VEGF ( E , H ) levels in hRECs ( C – E ) and MIO-M1 cells ( F – H ). In ( A , B ), data were analyzed by the formula 2 −ΔΔCT using β-actin as the internal standard and normalized to those measured in normoxic controls ( n = 3). In ( D , E , G , H ), β-actin was used as the loading control and OD values were normalized to those measured in normoxic controls ( n = 3). Data are shown as box plots with minimum to maximum whiskers. Statistical significance was evaluated through unpaired t -test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p > 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: Hypoxia-Dependent Upregulation of VEGF Relies on β3-Adrenoceptor Signaling in Human Retinal Endothelial and Müller Cells

    doi: 10.3390/ijms26094043

    Figure Lengend Snippet: Effect of 24 h hypoxia on hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) expression. ( A , B ) mRNA levels of VEGF in hRECs ( A ) and MIO-M1 cells ( B ). ( C – H ) Representative Western blots ( C , F ) and densitometric analysis ( D , E , G , H ) of HIF-1α ( D , G ) and VEGF ( E , H ) levels in hRECs ( C – E ) and MIO-M1 cells ( F – H ). In ( A , B ), data were analyzed by the formula 2 −ΔΔCT using β-actin as the internal standard and normalized to those measured in normoxic controls ( n = 3). In ( D , E , G , H ), β-actin was used as the loading control and OD values were normalized to those measured in normoxic controls ( n = 3). Data are shown as box plots with minimum to maximum whiskers. Statistical significance was evaluated through unpaired t -test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p > 0.0001.

    Article Snippet: hRECs constitute a primary human cell line composed of a mixture of venous and arterial cell populations [ ]. hRECs were cultured in Endothelial Basal Medium-2 (EBM-2; Lonza, Walkersville, MD, USA), supplemented with 10% Fetal Bovine Serum (FBS) and endothelial growth factors (Microvascular Endothelial Cell Growth Medium-2, EGM-2MV SingleQuot; Lonza).

    Techniques: Expressing, Western Blot, Control